The Primer Resuspension Calculator is a valuable tool used in molecular biology laboratories to determine the volume of solvent needed to achieve a desired concentration of primer solution. This calculator streamlines the process, saving researchers time and ensuring accuracy in their experiments.
Formula of Primer Resuspension Calculator
The calculation performed by the Primer Resuspension Calculator is based on the following formula:
Volume of solvent (in μL) = Desired primer concentration (in μM) * Primer mass (in μg) / Primer stock concentration (in μM)
Variables:
- Desired primer concentration: The concentration at which you want the primer to be resuspended (in micromolar).
- Primer mass: The mass of the primer powder available (in micrograms).
- Primer stock concentration: The concentration of the primer stock solution (in micromolar).
Table of General Terms
Here are some general terms related to primer resuspension that people often search for:
| Desired Primer Concentration (µM) | Dilution Factor (Stock Concentration / Desired Concentration) |
|---|---|
| 25 | 4 |
| 10 | 10 |
| 5 | 20 |
| 1 | 100 |
Note: This table provides a general guideline and may not apply to all primer stock concentrations. Always refer to the specific information provided with your primers.
Example of Primer Resuspension Calculator
Let’s consider an example to understand how the Primer Resuspension Calculator works:
Suppose we have a primer with a mass of 50 μg and a stock concentration of 100 μM. We want to resuspend it in a solution with a concentration of 10 μM.
Using the formula:
Volume of solvent (in μL) = 10 μM * 50 μg / 100 μM = (10 * 50) / 100 = 5 μL
Therefore, we need to add 5 μL of solvent to achieve the desired concentration of 10 μM.
Most Common FAQs
To use the Primer Resuspension Calculator, simply input the desired primer concentration, primer mass, and primer stock concentration into the respective fields. Then click the “Calculate” button to obtain the volume of solvent required.
Yes, the calculator accepts inputs in micromolar (μM) for concentrations and micrograms (μg) for mass. Ensure consistency in units to obtain accurate results.
Yes, the calculator is applicable to all types of primers, regardless of sequence or application.
