IDT Dilution Calculator Online

The IDT Dilution Calculator simplifies the task of diluting solutions to desired concentrations. It is an indispensable tool in laboratories, where precise concentrations of chemicals are necessary for successful experiments. By inputting a few simple parameters, the calculator ensures that users achieve accurate dilution ratios, thereby enhancing the reliability of their experimental results.

Formula of IDT Dilution Calculator

The cornerstone of the IDT Dilution Calculator is the formula:

This equation helps in calculating the volume and concentration adjustments required for dilutions:

• C1 represents the initial concentration of the primer stock solution (in mol/L or M).
• V1 denotes the volume of primer stock solution to be diluted (in L).
• C2 is the final desired concentration of the diluted primer solution (in mol/L or M).
• V2 is the final volume of the diluted primer solution (in L).

For practical applications, if you know the desired final volume and need to find the final concentration, the formula rearranges to:

C2 = (C1 * V1) / V

Practical Application Table

Initial Concentration (C1)	Final Desired Concentration (C2)	Initial Volume (V1)	Final Volume (V2)	Volume of Primer Needed (V1)
10 mM	1 mM	1 L	10 L	1 L
20 mM	1 mM	500 mL	10 L	0.5 L
50 mM	2 mM	2 L	25 L	1 L
100 mM	0.5 mM	100 mL	20 L	0.1 L
200 mM	10 mM	1 L	20 L	0.1 L

Example of IDT Dilution Calculator

For instance, if you have a primer solution with a concentration of 10 mol/L and you need 1 L of a 1 mol/L solution, the calculator will indicate that you need to mix:

V1 = (C2 * V2) / C1 = (1 mol/L * 1 L) / 10 mol/L = 0.1 L

This calculation shows that 0.1 L of the stock solution is needed to achieve the desired concentration.

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