Total Nucleotides: 0
Adenine (A): 0 (0.00%)
Thymine (T): 0 (0.00%)
Guanine (G): 0 (0.00%)
Cytosine (C): 0 (0.00%)
GC Content: 0.00%
AT Content: 0.00%
Purine Content (A+G): 0.00%
Pyrimidine Content (C+T): 0.00%
CpG O/E Ratio: 0.00
A DNA Percentage Calculator is a tool designed to analyze the composition of a DNA sequence by calculating the percentage of each nucleotide (Adenine, Thymine, Guanine, and Cytosine). It also helps in determining GC content, AT content, purine and pyrimidine composition, and CpG observed/expected ratios. This calculator is essential for genetic research, bioinformatics, and understanding DNA characteristics.
Formula of DNA Percentage Calculator
Nucleotide Percentage
Percentage of Nucleotide X = (Count of Nucleotide X / Total Number of Nucleotides) × 100Where X is A, T, G, or C (Adenine, Thymine, Guanine, or Cytosine).
GC Content
GC Content (%) = ((Count of G + Count of C) / Total Number of Nucleotides) × 100AT Content
AT Content (%) = ((Count of A + Count of T) / Total Number of Nucleotides) × 100Purine Content
Purine Content (%) = ((Count of A + Count of G) / Total Number of Nucleotides) × 100Pyrimidine Content
Pyrimidine Content (%) = ((Count of C + Count of T) / Total Number of Nucleotides) × 100CpG Observed/Expected Ratio
CpG O/E Ratio = (Number of CpG × Total Number of Nucleotides) / (Count of C × Count of G)Where:
- Number of CpG is the count of CG dinucleotides in the sequence.
- Total Number of Nucleotides is the length of the sequence.
- Count of C is the total number of cytosine nucleotides.
- Count of G is the total number of guanine nucleotides.
General Terms Table
| Term | Definition |
|---|---|
| GC Content | Percentage of guanine and cytosine in DNA |
| AT Content | Percentage of adenine and thymine in DNA |
| CpG Ratio | Observed/Expected ratio of CpG dinucleotides |
| Purine Content | Percentage of purines (A and G) |
| Pyrimidine Content | Percentage of pyrimidines (C and T) |
Example of DNA Percentage Calculator
Let’s consider the DNA sequence: AGCTAGCTAGCTA
- Count of A = 4
- Count of T = 4
- Count of G = 3
- Count of C = 3
- Total Nucleotides = 14
Using the formulas:
- GC Content = ((3+3) / 14) × 100 = 42.86%
- AT Content = ((4+4) / 14) × 100 = 57.14%
- Purine Content = ((4+3) / 14) × 100 = 50%
- Pyrimidine Content = ((3+4) / 14) × 100 = 50%
Most Common FAQs
GC content represents the percentage of guanine and cytosine nucleotides in a DNA sequence. It is crucial for determining DNA stability and its melting temperature.
The CpG ratio is used in epigenetic studies to understand DNA methylation, which influences gene expression and regulation.
No, this calculator is designed specifically for DNA sequences. RNA sequences use uracil (U) instead of thymine (T).