The Copies Per µL Calculator helps quantify the concentration of a target sequence, such as DNA, RNA, or microorganisms, within a small sample volume measured in microliters (µL). This tool is frequently used in molecular biology, microbiology, and virology, particularly in experiments involving qPCR or RT-PCR.
By combining the number of detected copies, sample volume, and dilution factor, this calculator ensures accurate concentration results. Researchers and laboratory professionals rely on this tool for diagnostics, viral load analysis, and quantification in research studies.
Formula of Copies Per Ul Calculator
The formula for calculating copies per µL is:
Copies per µL = (Number of Copies Detected / Volume of Sample Tested in µL) × Dilution Factor
Detailed Formula Components:
- Number of Copies Detected: The total number of target copies identified in the tested sample, typically measured using qPCR or similar techniques.
- Volume of Sample Tested in µL: The volume of the tested sample, expressed in microliters (µL).
- Dilution Factor: A multiplier to account for any dilution of the sample before testing. If no dilution is applied, the dilution factor is 1.
Key Notes:
- Accurate measurement of all components is crucial for reliable results.
- Ensure the volume is recorded in µL for compatibility with the formula.
Pre-Calculated Values Table
This table provides reference values for common scenarios to simplify calculations:
| Number of Copies Detected | Volume of Sample (µL) | Dilution Factor | Copies per µL |
|---|---|---|---|
| 1,000 | 10 | 1 | 100 |
| 5,000 | 5 | 1 | 1,000 |
| 2,500 | 2 | 2 | 2,500 |
| 10,000 | 50 | 5 | 1,000 |
| 50,000 | 100 | 10 | 5,000 |
Example of Copies Per Ul Calculator
Scenario:
A laboratory technician tests a 5 µL sample and detects 12,000 copies of a target sequence. The sample was diluted by a factor of 2 before testing. Calculate the copies per µL.
Step-by-Step Solution:
- Identify Components:
- Number of Copies Detected = 12,000
- Volume of Sample Tested = 5 µL
- Dilution Factor = 2
- Apply the Formula: Copies per µL = (Number of Copies Detected / Volume of Sample Tested in µL) × Dilution Factor
Copies per µL = (12,000 / 5) × 2 - Perform the Calculation: Copies per µL = 2,400 × 2
Copies per µL = 4,800
Result:
The concentration of the target sequence is 4,800 copies per µL.
Most Common FAQs
This calculation helps quantify the concentration of target molecules in a small sample, ensuring accuracy in experiments such as qPCR, diagnostics, and research applications.
The dilution factor represents the ratio of the final sample volume to the original sample volume. For example, if 1 µL of the sample is dilute to 10 µL, the dilution factor is 10.
Yes, the calculator applies to any scenario requiring concentration determination for small sample volumes, including chemical and particulate quantifications.