Copies per mL: –
The Copies Per mL Calculator is a tool used to determine the concentration of a target molecule, such as DNA, RNA, or microorganisms, in a liquid sample. This calculation is commonly employed in fields like molecular biology, virology, and microbiology, especially in quantitative PCR (qPCR) or RT-PCR experiments.
By combining test results, sample volume, and dilution factor, the calculator provides accurate and reproducible values for sample concentrations. This tool is essential for researchers, clinicians, and laboratory professionals working on diagnostic and research applications.
Formula of Copies Per Ml Calculator
The formula for calculating copies per mL is:
Copies per mL = (Number of Copies Detected / Volume of Sample Tested) × Dilution Factor
Detailed Formula Components:
- Number of Copies Detected: The total number of target copies identified in the tested sample, typically measured using qPCR or similar techniques.
- Volume of Sample Tested: The volume of the sample used for testing, usually measured in milliliters (mL).
- Dilution Factor: A multiplier that accounts for any dilution of the sample before testing. If no dilution is applied, the dilution factor is 1.
Important Notes:
- Accurate measurement of all components is critical for reliable results.
- Dilution adjustments are necessary to account for pre-processing steps in experiments.
Pre-Calculated Values Table
This table provides examples of typical copies per mL values for various sample scenarios:
| Number of Copies Detected | Volume of Sample (mL) | Dilution Factor | Copies per mL |
|---|---|---|---|
| 10,000 | 1.0 | 1 | 10,000 |
| 25,000 | 0.5 | 1 | 50,000 |
| 5,000 | 0.2 | 10 | 250,000 |
| 15,000 | 0.3 | 5 | 250,000 |
| 50,000 | 1.0 | 2 | 100,000 |
Example of Copies Per Ml Calculator
Scenario:
A laboratory technician tests a 0.5 mL sample and detects 12,000 copies of a target sequence. The sample was diluted by a factor of 2 before testing. Calculate the copies per mL.
Step-by-Step Solution:
- Identify Components:
- Number of Copies Detected = 12,000
- Volume of Sample Tested = 0.5 mL
- Dilution Factor = 2
- Apply the Formula: Copies per mL = (Number of Copies Detected / Volume of Sample Tested) × Dilution Factor
Copies per mL = (12,000 / 0.5) × 2 - Perform the Calculation: Copies per mL = 24,000 × 2
Copies per mL = 48,000
Result:
The concentration of the target sequence is 48,000 copies per mL.
Most Common FAQs
This calculation helps determine the concentration of a specific target in a liquid sample, enabling accurate diagnostics, viral load estimation, or microbial quantification.
The dilution factor is the ratio of the final volume of the diluted sample to the initial volume of the undiluted sample. For example, if 1 mL of sample is dilute to 10 mL, the dilution factor is 10.
Yes, the formula applies to any scenario requiring concentration calculations, such as chemical solutions or particulate matter in liquid samples.