This calculator helps researchers determine the exact amount of water needed to resuspend lyophilized oligonucleotides to a specific concentration. It ensures that the oligonucleotides are at the correct concentration for subsequent experiments, which is critical for the accuracy and reproducibility of results.
Formula of IDT Resuspension Calculator
To calculate the water volume required, apply the formula:
Here, Water volume (µL) is the volume of water needed for resuspension, Amount of oligonucleotide (nmol) represents the total amount of dried oligonucleotide, and Desired concentration (µM) is the target concentration for the resuspended solution. This formula ensures that researchers can achieve the precise concentration needed for their work.
Table of Typical Values
| Amount of Oligonucleotide (nmol) | Desired Concentration (µM) | Water Volume (µL) |
|---|---|---|
| 100 | 10 | 10000 |
| 50 | 20 | 2500 |
| 20 | 50 | 400 |
This table provides quick reference values for common concentrations and oligonucleotide amounts, facilitating easier calculations.
Example of IDT Resuspension Calculator
To make the concepts even more clear let us look at a detailed example. Consider resuspending 50 nmol of oligonucleotide to achieve a concentration of 20 µM. Using the formula:
Water volume (µL) = (50 nmol * 1000) / 20 µM = 2500 µL
This example illustrates how to use the calculator to determine the necessary volume of water for resuspension.
Most Common FAQs
An oligonucleotide is a small DNA or RNA molecule that is often used in research, diagnostics, and therapeutic applications.
Accurate resuspension ensures that the oligonucleotide concentrations are precise, which is critical for the validity and reproducibility of experimental results.
The IDT Resuspension Calculator saves time and reduces errors in preparing oligonucleotides, enhancing experimental efficiency and reliability.